Identification of loci and candidate genes controlling kernel weight in barley based on a population for which whole genome assemblies are available for both parents

Kernel weight (KW), together with kernel number per unit area, determines yield of cereal crops. Here, two barley recombinant inbred lines (RILs) populations a shared parent were used to identify loci controlling KW. One is Baudin/AWCS276 (BA) for which linkage map was available. Several large-effect QTL KW detected in this population. Another Morex/AWCS276 (MA). A 5273 makers formed 1454 clusters, constructed by the genotyping sequence (GBS) data 201 RILs from single marker selected represent each clusters and these markers covers total length 1022.4 cM an average interval approximately 0.7 between loci. Three (located on 2HL, 6HL, 7HL, respectively) identified BA population also MA under different environments. The locus 6HL experiments conducted both thus developing near isogenic (NILs). Apart KW, isolines pair putative NILs obtained showed no significant difference any morphological characteristics assessed. all about 15% based assessments glasshouse field conditions. Taken advantage that high quality genome assemblies Morex AWCS276 are available, we candidate genes underlying three orthologous analysis. developed study should facilitate cloning functional analysis regulating barley.